N-Hexyl-0-glucosyl sphingosine, an inhibitor of glucosyl ceramide, β-glucosidase
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چکیده
منابع مشابه
The effects of N-hexyl-O-glucosyl sphingosine on normal cultured human fibroblasts: a chemical model for Gaucher's disease.
Normal human skin fibroblasts were grown in the presence of N-hexyl-O-glucosyl sphingosine (HGS), an inhibitor of aryl glucosidase and glucocerebrosidase. Tests of the cells with aryl glycosides showed that beta-glucosidase activity in the cells was drastically reduced while other enzyme activities (alpha-glucosidase, beta-galactosidase, and N-acetyl-beta-hexosaminidase) were normal or elevated...
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An improved method for isolating cerebrosides from natural sources is described. The method is particularly suited to large scale work and can be adapted to the isolation of sphingolipids that are less polar than the gangliosides. It is based on the use of sodium sulfate to absorb the water from chloroform-methanol tissue extracts, the use of triiodide to cleave the ether linkage of plasmalogen...
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In the crystal structure of the title compound, C(21)H(25)NO(11), the hexopyranosyl ring adopts a chair conformation and the five substituents are in equatorial positions. An intra-molecular hydrogen bond between the amino group and a neighbouring carbonyl group is found. Two carbonyl groups are disordered and were refined using a split model.
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Since xylose could be substituted for glucose to yield 4-0-a-Dglucosyl n-xylose (a), it seemed possible that the enzyme could be used for the synthesis of yet other disaccharides possessing the a-l,4 linkage. The present communication demonstrates that glucosamine, N-acetylglucosamine, and 2-deoxyglucose can indeed serve as substrates to yield the corresponding maltose analogues. The preparatio...
متن کاملSynthesis of galactosyl ceramide and glucosyl ceramide by rat brain: assay procedures and changes with age.
Galactosyl ceramide is made in mouse brain from ceramide and uridine diphospho galactose (UDPGal)16,1L The enzyme system, evaluated in vitro, shows a marked preference for 2-hydroxy fatty acids (HFA) ceramide when compared with nonhydroxy fatty acids (NFA) ceramide, although brain contains HFA and NFA cerebroside in similar amounts. We do not yet know whether this difference is due to enzyme sp...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1973
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)36899-1